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Arkistoitu opetussuunnitelma 2015–2017
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BIO4701 SGN-53806 Techniques in Molecular Biology and Applications to Gene Expression 3 op
Organised by
Master's Degree Programme in Bioinformatics

General description

Course organized by TUT, see TUT study guide for up-to-date information.

Learning outcomes

After the course, the student can:
- Identify, list and define common techniques used in genetic engineering and gene expression analysis,
- Interpret data generated from the techniques discussed, classify strengths and weaknesses of the methodologies, summarize results and methods, infer consequences of results, explain procedures.
- Apply the acquired knowledge to better interpret degrees of confidence in experimental results.
- Deconstruct results of gene expression measurements. Compare different methodologies for verifying a hypothesis or measuring a variable.
- Compare and appraise related methods in gene expression studies, and interpret and comment conclusions in such studies,
- Create and develop an experimental procedure using the methods learned, that can address simple questions regarding gene expression.

Contents

- The structure, design and applicability of the bacterial plasmid vector to clone a gene of interest and express protein.
- The introduction of genomic insertion by homologous recombination.
- Studies of bacterial gene expression.
- Studies of variability in bacterial gene expression at the single cell level, with an emphasis on cell-to-cell variation.
- Application and analysis of PCR method.
- The theory and practice of fluorescence microscopy, as used to test for protein expression and localization.

Modes of study

Option 1
Available for:
  • Degree Programme Students
  • Other Students
  • Open University Students
  • Doctoral Students
  • Exchange Students
Participation in course work 
In English

Evaluation

Numeric 1-5.

Belongs to following study modules

BioMediTech
2015–2016
Teaching
Archived Teaching Schedule. Please refer to current Teaching Shedule.
BioMediTech