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Arkistoitu opetusohjelma 2018–2019
Selaat vanhentunutta opetusohjelmaa. Voimassa olevan opetusohjelman löydät täältä.
BTK4321 Protein technology Laboratory Course A 7 ECTS
Periods
Period I Period II Period III Period IV
Language of instruction
English
Type or level of studies
Advanced studies
Course unit descriptions in the curriculum
Bioteknologian tutkinto-ohjelma
Faculty of Medicine and Life Sciences

Learning outcomes

After completing the course, the student is able to:
-apply DNA mutagenesis
-perform DNA sequencing
-produce recombinant proteins
-purify proteins using chromatorgaphic methods
-analyze proteins using electrophoretic methods
-utilize recombinant proteins and mutagenesis as a research tool

General description

Laboratory course focusing on recombinant DNA technology and protein research.

During the laboratory course we prepare recombinant DNA plasmids encoding for recombinant proteins and their mutants. We produce the proteins in E. coli, purify them using affinity chromatography, and analyze the proteins using electrophoresis and Western blot.

Enrolment for University Studies

Enrolment time has expired

Teachers

Vesa Hytönen, Teacher responsible
Vesa.Hytonen[ät]uta.fi

Teaching

6-May-2019 – 17-May-2019
Tutorials 80 hours
Laboratory work 6.5.-17.5.2019
Independent work 80 hours

Evaluation

Numeric 1-5.

Evaluation criteria

Laboratory report 50%

Laboratory work performance 25%

Exam 25%